In this lesson you will learn how enzymes speed up metabolic reactions and how they are regulated.
By the end of this lesson, students should be able to:
- explain an energy profile for a chemical reaction using a free energy diagram
- define activation energy, activation energy barrier, substrate specificity, induced-fit, allosteric regulation, inhibition, feedback inhibition and cofactor.
- explain how temperature and pH affect enzyme activity.
- analyze a feedback inhibition of a biochemical pathway and then predict what the result of inhibiting an enzyme in an early step does to the overall pathway and the production of the end-product.
- determine the effect of pH and substrate concentration on enzyme activity using a virtual lab.
Let’s start by examining the environmental factors affect enzyme activity and then allosteric regulation of enzymes in the video lesson below. Please take notes as you watch the video lesson.
In order to assess the effects of concentration and pH on enzyme activity you will conduct a virtual lab. Please print out the pdf file (homework) below before conducting the experiment. Follow instructions closely as outlined in the lab below. To access the virtual lab, click on the link below.
Once you complete your lab, please answer all questions, scan your document and save it as a pdf file. Next, you will upload or e-mail it to us for grading. Have fun conducting this interesting virtual lab.
A key skill in biology is the mastering of new terms and their definitions. Please print out and complete the crossword puzzle below on the molecules of life. Once completed, either upload or e-mail it to us. The answers to 8 and 14 across are: open and restriction.