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Gene Expression Test

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Gene Expression Test

Biology 12 (SBI4U) Unit 3 – Molecular Genetics Gene Expression Test

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Question 1 of 28
1. Question
1 point(s)
Category: K/U
1. What term describes the synthesis of mRNA from a DNA template?
- a) Initiation
- b) Transcription
- c) Translation
- d) Termination
- e) Thymine dimer repair
Correct
Incorrect
Question 2 of 28
2. Question
1 point(s)
Category: K/U
2. The arrangement of bases in a molecule of mRNA is best described as
- a) Branched
- b) Linear
- c) Paired
- d) Linked into a beta-pleated sheet
- e) Circular
Correct
Incorrect
Question 3 of 28
3. Question
1 point(s)
Category: K/U
3. Where does the RNA polymerase complex bind to initiate transcription?
- a) The start codon
- b) The operator
- c) The promoter
- d) The precursor mRNA
- e) The genes for metabolizing lactose
Correct
Incorrect
Question 4 of 28
4. Question
1 point(s)
Category: K/U
4. Which of the following correctly describes the order in which cell components become involved in protein synthesis?
- a) DNA polymerase, mRNA, ribosome, tRNA
- b) mRNA, RNA polymerase, ribosome, tRNA
- c) RNA polymerase, mRNA, tRNA, ribosome
- d) RNA polymerase, mRNA, ribosome, tRNA
- e) DNA, rRNA, tRNA, mRNA
Correct
Incorrect
Question 5 of 28
5. Question
1 point(s)
Category: K/U
5. Identify the tRNA anticodon that corresponds with the mRNA codon 5′-GAA-3′
- a) 5'-GAA-3'
- b) 3'-GAA-5'
- c) 5'-CTT-3'
- d) 3'-CTT-5'
- e) 3'CUU-5'
Correct
Incorrect
Question 6 of 28
6. Question
1 point(s)
Category: K/U
6. Transcription of the lacZ and lacY genes of the lac operon begins when
- a) lactose binds to the operator.
- b) RNA polymerase binds to the lacZ gene.
- c) the repressor protein binds to the inducer.
- d) the repressor is released from the promoter
- e) lactose binds to RNA polymerase.
Correct
Incorrect
Question 7 of 28
7. Question
1 point(s)
Category: K/U
7. The expression of the repressor (lacl) gene, which is part of the lac operon
- a) occurs only when RNA polymerase binds to the promoter.
- b) causes lactose to pass through the cell membrane.
- c) occurs only when lactose is present.
- d) results in the removal of the repressor from the operator.
- e) is part of the negative control mechanism of the lacZ and lacY genes.
Correct
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Question 8 of 28
8. Question
1 point(s)
Category: K/U
8. In eukaryotes, introns are removed before mRNA leaves the nucleus because
- a) they do not code for protein.
- b) they prevent the movement of ribosomes.
- c) they prevent the binding of ribosomes to mRNA.
- d) the mRNA would be too long to pass through the nuclear pores if the introns remained in it.
- e) they do not consist of the same bases as the rest of the mRNA.
Correct
Incorrect
Question 9 of 28
9. Question
1 point(s)
Category: K/U
9. RNA polymerase participates in
- a) DNA replication
- b) RNA replication
- c) DNA transcription
- d) RNA transcription
- e) RNA translation
Correct
Incorrect
Question 10 of 28
10. Question
1 point(s)
Category: K/U
10. Base pairing due to hydrogen bonding is fundamental to
- a) the process of DNA transcription, but not to the process of DNA replication.
- b) the process of DNA replication. but not to the process of DNA transcription.
- c) neither process.
- d) both processes.
- e) mitosis, but not DNA transcription
Correct
Incorrect
Question 11 of 28
11. Question
1 point(s)
Category: K/U
The parts of genes that are transcribed into mRNA but are later removed are called
- a) exons.
- b) spliceosomes.
- c) introns.
- d) peptides.
- e) mutations.
Correct
Incorrect
Question 12 of 28
12. Question
1 point(s)
Category: K/U
12. Translation is a process in which a polypeptide is produced from mRNA. What is the role of the tRNA in this process?
- a) It carries a particular amino acid to the correct codon site..
- b) It stores genetic information.
- c) It carries genetic information from DNA.
- d) It provides information to a ribosome.
- e) It proofreads the growing polypeptide.
Correct
Incorrect
Question 13 of 28
13. Question
1 point(s)
Category: K/U
13. The triplet of nucleotides in tRNA that is complementary to a triplet of nucleotides in mRNA is called a(n)
- a) codon.
- b) anticodon.
- c) ribosome.
- d) genetic code.
- e) sequence.
Correct
Incorrect
Question 14 of 28
14. Question
1 point(s)
Category: K/U
14. An amino acid is
- a) part of an mRNA.
- b) a monomer.
- c) part of a nucleic acid.
- d) a protein.
- e) a basic unit of a carbohydrate.
Correct
Incorrect
Question 15 of 28
15. Question
1 point(s)
Category: T
15. The splicing of the first-formed mRNA transcript by spliceosomes is necessary because
- a) the poly-A tail must be added.
- b) introns need to be added.
- c) the poly-A tail and the 5'-cap must be removed.
- d) the transcript must be freed from the DNA.
- e) introns need to be removed.
Correct
Incorrect
Question 16 of 28
16. Question
2 point(s)
Category: T
16. The repressor protein of the lac operon has two different recognition and binding sites for other molecules. Which of the following pairs of molecules do you think it likely that these sites would bind?
- a) RNA polymerase and lactose
- b) RNA polymerase and DNA
- c) a DNA sequence on the operator and lactose
- d) a DNA sequence on the regulator gene and lactose
- e) lactose and DNA polymerase
Correct
Incorrect
Question 17 of 28
17. Question
2 point(s)
Category: T
17. In the presence of high cellular concentrations of tryptophan
- a) the repressor attaches to the DNA, but transcription proceeds.
- b) the repressor binds to tryptophan and then leaves the operator.
- c) tryptophan binds to the operator and prevents transcription.
- d) tryptophan binds to the repressor, which then binds to the operator.
- e) tryptophan binds to the repressor, which binds to the promoter and prevents transcription.
Correct
Incorrect
Question 18 of 28
18. Question
3 point(s)
Category: T
18. Which of the following is true with respect to the lac operon?
- a) The repressor only binds to the operator when it is bound to the product of the enzymes coded for by the genes of the operon.
- b) The repressor only binds to the operator when it is bound to the mRNA transcript of the lac Z and lac Y genes.
- c) The repressor only leaves the operator when it is bound to the protein product of the lac I gene.
- d) The repressor is always bound to the operator, but changes shape when lactose binds to it, allowing RNA polymerase to pass.
- e) The repressor is bound to the operator, except when it is bound to the inducer (allolactose).
Correct
Incorrect
Question 19 of 28
19. Question
3 point(s)
Category: T
19. Which of the following mutations would be most likely to have a harmful effect on an organism?
- a) A base-pair substitution
- b) A deletion of three nucleotides near the middle of the gene
- c) A single nucleotide deletion in the middle of an intron.
- d) A single nucleotide deletion near the end of the coding sequence.
- e) A single nucleotide insertion downstream of, and close to, the start of the coding sequence
Correct
Incorrect
Question 20 of 28
20. Question
2 point(s)
Category: T
20. Compare the two mRNA sequences below:

AUAUUCGGCAAUCCG
AUAUUCCGCAAUCCG

This change could be the result of a
- a) nonsense mutation.
- b) addition mutation.
- c) deletion mutation.
- d) translocation.
- e) point mutation.
Correct
Incorrect
Question 21 of 28
21. Question
2 point(s)
Category: T
21. The operator of the lac operon is ‘upstream’ of the genes, which codes for enzymes. ‘Upstream’ means
- a) the operator is in the first part of the lacZ gene.
- b) the operator is crossed by RNA polymerase before RNA polymerase reaches the genes, which code for enzymes.
- c) the operator is crossed by RNA polymerase before RNA polymerase crosses the promoter.
- d) the operator is separated from the lacZ gene by thousands of bases.
- e) an intron separates the operator and the genes, which code for enzymes.
Correct
Incorrect
Question 22 of 28
22. Question
2 point(s)
Category: T
22. The function of the polyadenine tail that is added to mRNA in eukaryotic cells is to
- a) prevent degradation of the mRNA.
- b) initiate the binding of the mRNA to the ribosome.
- c) help the mRNA to bind to a pore in the nuclear membrane.
- d) add a specific amino acid sequence to the polypeptide produced.
- e) act as a signal for the termination of translation.
Correct
Incorrect
Question 23 of 28
23. Question
2 point(s)
Category: T
23. A certain protein needs to have a high energy phosphate group added to it in the cytoplasm before it becomes active. This offers the possibility of control at which of the following levels?
- a) Transcriptional
- b) Operational
- c) Post-transcriptional
- d) Translational
- e) Post-translational
Correct
Incorrect
Question 24 of 28
24. Question
6 point(s)
Category: C
24. Briefly describe the function of transfer RNA.
Correct
Incorrect
Question 25 of 28
25. Question
5 point(s)
Category: C
25. The genetic code is called ‘degenerate’, meaning many amino acids are coded for by more than one codon. Why is this not surprising in view of the number of amino acids commonly used in protein synthesis?
Correct
Incorrect
Question 26 of 28
26. Question
6 point(s)
Category: A
26. What is the amino acid sequence of the peptide that would be synthesized after transcription and translation of the following piece of template strand DNA? You should note that the template strand of the DNA and the newly-synthesized mRNA strand will be ‘anti-parallel’ (i.e., their 5′ ends will be at opposite ends of the strands of nucleic acid.)

5′ 3′ Codon translations
______________________ codon amino acid
T C A T G C G C A A C A
AGU
Ser
ACG Thr
CGU Arg
UGU Cys
UGC Cys
GCA Ala
UGA Stop
Correct
Incorrect
Question 27 of 28
27. Question
5 point(s)
Category: A
26. The cells of the lily plant have eighteen times more DNA than human cells, yet human DNA is more complex. Explain this phenomenon
Correct
Incorrect
Question 28 of 28
28. Question
5 point(s)
Category: A
28. A mutation occurs in the promoter of a eukaryotic gene, eliminating the TATA box. How will this mutation affect the transcription of this gene?
Correct
Incorrect

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